Auflistung nach Schlagwort "Western blotting"

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  • Gold Nanoparticle Mediated Laser Transfection for Efficient siRNA Mediated Gene Knock Down 
    Heinemann, Dag; Schomaker, Markus; Kalies, Stefan; Schieck, Maximilian; Carlson, Regina; Murua Escobar, Hugo; Ripken, Tammo; Meyer, Heiko; Heisterkamp, Alexander (San Francisco, California, US : PLOS, 2013)
    Laser based transfection methods have proven to be an efficient and gentle alternative to established molecule delivery methods like lipofection or electroporation. Among the laser based methods, gold nanoparticle mediated ...
  • Hydrogel-based microfluidics for vascular tissue engineering 
    Koroleva, Anastasia; Deiwick, Andrea; Nguyen, Alexander; Narayan, Roger; Shpichka, Anastasia; Kufelt, Olga; Kiyan, Roman; Bagratashvili, Victor N.; Timashev, Peter; Scheper, Thomas; Chichkov, Boris (Berlin : De Gruyter, 2016)
    In this work, we have explored 3-D co-culture of vasculogenic cells within a synthetically modified fibrin hydrogel. Fibrinogen was covalently linked with PEG-NHS in order to improve its degradability resistance and ...
  • A potential late stage intermediate of twin-arginine dependent protein translocation in Escherichia coli 
    Geise, Hendrik; Heidrich, Eyleen Sabine; Nikolin, Christoph Stefan; Mehner-Breitfeld, Denise; Brüser, Thomas (Lausanne : Frontiers Media S.A., 2019)
    The twin-arginine translocation (Tat) system transports folded proteins across membranes of prokaryotes, plant plastids, and some mitochondria. According to blue-native polyacrylamide gel electrophoresis after solubilization ...
  • Precursor B-ALL cell lines differentially respond to syk inhibition by entospletinib 
    Sender, Sina; Sekora, Anett; Perez, Simon V.; Chabanovska, Oleksandra; Becker, Annegret; Ngezahayo, Anaclet; Junghanss, Christian; Murua Escobar, Hugo (Basel : MDPI, 2021)
    Background: Impaired B-cell receptor (BCR) function has been associated with the pro-gress of several B-cell malignancies. The spleen tyrosine kinase (SYK) represents a potential therapeutic target in a subset of B-cell ...
  • Regulatory coiled-coil domains promote head-to-head assemblies of AAA+ chaperones essential for tunable activity control 
    Carroni, Marta; Franke, Kamila B.; Maurer, Michael; Jäger, Jasmin; Hantke, Ingo; Gloge, Felix; Linder, Daniela; Gremer, Sebastian; Turgay, Kürsad; Bukau, Bernd; Mogk, Axel (Cambridge : eLife Sciences Publications, 2017)
    Ring-forming AAA+ chaperones exert ATP-fueled substrate unfolding by threading through a central pore. This activity is potentially harmful requiring mechanisms for tight repression and substrate-specific activation. The ...
  • Structural Basis for Regulation of the Opposing (p)ppGpp Synthetase and Hydrolase within the Stringent Response Orchestrator Rel 
    Pausch, Patrick; Abdelshahid, Maha; Steinchen, Wieland; Schäfer, Heinrich; Gratani, Fabio Lino; Freibert, Sven-Andreas.; Wolz, Christiane; Turgay, Kürşad; Wilson, Daniel N.; Bange, Gert (New York, NY : Elsevier, 2020)
    The stringent response enables metabolic adaptation of bacteria under stress conditions and is governed by RelA/SpoT Homolog (RSH)-type enzymes. Long RSH-type enzymes encompass an N-terminal domain (NTD) harboring the ...